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To change normality, the ratio data was indeed transformed just before analyses

To change normality, the ratio data was indeed transformed just before analyses

A standardized, cumulative index of cross-fitness is computed for every single combination of maternal forest and you will pollen donor, considering adult good fresh fruit place, seeds germination, and you will survivorship and development of seedlings. For every single types, mixed-design data of variance was utilized to assess the effects of crossing procedures (fixed effect; which have maternal forest integrated once the a random perception) towards the percentage of hand-pollinated herbs function adult fruits meilleurs sites de rencontres thaïlandais, pricing away from seeds germination and seedling survivorship, seedling size during the step 1 yr, and collective physical fitness. Multiple patterns was indeed tested playing with ANOVA: (a) also all the treatments, (b) leaving out unbalanced services, to allow review of communications conditions, (c) minus selfing cures (as maternal woods was basically largely otherwise totally notice-incompatible), and (d) grouping the in this-Sinharaja outcrossing treatments to check on the effect from within- against. between-forest crossing. The result away from crossing range on each factor is actually next tested using linear or quadratic regression investigation, with respect to the model of the relationship. Lastly, for each maternal tree, the consequences regarding nearby-neighbor and you will enough time-distance mating was in fact estimated courtesy indicator of biparental inbreeding despair and outbreeding despair, correspondingly, according to cumulative physical fitness values.

Syzygium rubicundum

Fruit abortion was heavy for all trees, resulting in low fruit set (range across treatments: 2.0–9.7%; Fig. 2a). The timing of abortion was not discernable across treatments. Self-compatibility was low, but variable, across maternal trees (Fig. 2a). Flowers used for tests of apomixis (N = 360) and autogamy (N = 582) failed to set fruit. All analyses of variance in fruit set revealed a highly significant treatment effect and significant maternal tree effect, but no significant interaction between treatment and maternal tree (Tables 2A and 3A). For all three trees, the percentage of experimental flowers setting mature fruit showed a consistent increase with crossing distance, followed by a severe decline in fruit set with the distant between-forest treatment (Fig. 2a). The relationship between crossing distance and fruit set was nearly identical for the three maternal trees and significant with or without the self-pollinated treatment included in the model (quadratic regression model: arcsine square-root [fruit set] = crossing distance [km] + crossing distance 2 ; results without self-pollinated treatment: F2,57 = 8.25, P < 0.0007, R 2 = 0.47). Peak mean fruit set occurred at a crossing distance of 1–2 km (distant within-forest treatment) and was 1.7–4.7 times greater than mean fruit set rates for other hand-pollination treatments, averaged across maternal trees. Mean fruit set rate for the distant within-forest treatment was significantly greater than those for all treatments except distant-neighbor and open-pollinated, but consistently exceeded fruit set of open-pollinated flowers (Fig. 2a).

Shorea cordifolia

Fruit set was also low for Sh. cordifolia (range across treatments: 0–5.3%; Fig. 2b). Again, the timing of fruit abortion was not discernable among treatments. Selfed and distant between-forest treatments resulted in 0% and <1% fruit set, respectively. Fruit set from the intermediate-distance cross-pollinations varied across maternal trees, but with one exception (nearest-neighbor treatment at Tree number 1) indicated optimal fruit set at an outcrossing range of ?2 km (distant neighbor treatment; Fig. 2b). All analyses of variance in fruit set revealed a highly significant treatment effect, but no maternal tree effect (Tables 2B and 3B). The relationship between crossing distance and fruit set was significant only when the selfed treatment was excluded (quadratic regression model: arcsin square root [fruit set] = crossing distance [km] + crossing distance 2 ; F2,57 = 5.71, P < 0.006, R 2 = 0.41). At each maternal tree, fruit set rate for open-pollinated flowers was greater than that for all hand-cross treatments, suggesting that some aspect of the hand-pollination procedure (e.g., flower handling, bagging) caused reduced fruit set in Sh. cordifolia.



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